negative kit control  (Zymo Research)

Journal: Communications biology

Article Title: Best practices for methylome characterization in novel species: a case study in the microalgae Microchloropsis.

doi: 10.1038/s42003-025-08027-6

Figure Lengend Snippet: Fig. 2 | The percent 5-methylcytosine (%5mC) DNA methylation in 5-azadeoxycytidine-treated M. salina cultures is negligible. a The calibration curve for %5mC DNA methylation was generated from standards with known quantities of DNA methylation from the Zymo 5mC DNA methylation ELISA kit according to the manufacturer’s instruc- tions. Standard deviations of triplicate measure- ments are shown. b Multiple aliquots (shown as dots) from the M. salina cultures from the pilot study (Fig. 1a) were used to assess the %5mC DNA methylation in response to 5AZA treatment. There were no statistical differences between measure- ments taken of the negative (−) control, 20 µM 5AZA, 40 µM 5AZA, and 80 µM 5AZA samples (as individual replicates). Aliquots from the repeated experiment (Fig. 1b) (separated by the dotted line) were also assessed (DMSO control and 80 µM 5AZA_2 only). They also showed no statistical dif- ferences from the negative (−) control.

Article Snippet: Variability in the Enzo ELISA was much greater than the Zymo ELISA kit (Fig. 2) for the (+) control and the M. salina samples.

Techniques: DNA Methylation Assay, Generated, Enzyme-linked Immunosorbent Assay, Negative Control, Control

Journal: Communications biology

Article Title: Best practices for methylome characterization in novel species: a case study in the microalgae Microchloropsis.

doi: 10.1038/s42003-025-08027-6

Figure Lengend Snippet: Fig. 3 | The percent 5-methylcytosine (%5mC) DNA methylation in M. salina spinner cultures is negligible. M. salina was cultivated in 1 L spinner flasks; cultures were not treated with 5-azadeoxycytidine as done in the previous experi- ments. a Calibration curve for %5mC DNA methylation generated from 100 ng gDNA stan- dards with known quantities of DNA methylation from the Enzo 5mC DNA methylation ELISA kit. b %5mC DNA methylation data from 100 ng gDNA M. salina samples from four spinner cultivation flasks. c Calibration curve for %5mC DNA methy- lation generated from 200 ng gDNA standards with known quantities of DNA methylation from the Enzo 5mC DNA methylation ELISA kit. d %5mC DNA methylation data from 200 ng gDNA M. salina samples from four spinner cultivation flasks. Mea- surements from the four M. salina cultures were not statistically different from the (−) control readings at either concentration: 100 ng (b) nor 200 ng (d) of gDNA. Variability in the Enzo ELISA was much greater than the Zymo ELISA kit (Fig. 2) for the (+) control and the M. salina samples.

Article Snippet: Variability in the Enzo ELISA was much greater than the Zymo ELISA kit (Fig. 2) for the (+) control and the M. salina samples.

Techniques: DNA Methylation Assay, Generated, Enzyme-linked Immunosorbent Assay, Control, Concentration Assay

Journal: BMC cancer

Article Title: ACSL4 promotes the formation of the proliferative subtype in hepatoblastoma.

doi: 10.1186/s12885-025-13592-4

Figure Lengend Snippet: Fig. 4 ACSL4 promotes the proliferation of hepatoblastoma cells in vitro and tumor growth in vivo. (A) The viability of HepG2 and Huh6 cells transfected with siACSL4 or NC was detected by CCK8 assays. (B) Colony formation assays were performed in ACSL4-silenced and control cells. *p < 0.05, **p < 0.01, ***p < 0.001

Article Snippet: Total RNA was extracted from ACSL4 knockdown and negative control lentivirus-infected HepG2 cells using the RNAstorm Fresh Cell and Tissue RNA Isolation Kit from Cell Data Sciences (USA).

Techniques: In Vitro, In Vivo, Transfection, Control

Journal: BMC cancer

Article Title: ACSL4 promotes the formation of the proliferative subtype in hepatoblastoma.

doi: 10.1186/s12885-025-13592-4

Figure Lengend Snippet: Fig. 6 ACSL4 promotes the formation of proliferative HB subtype via MAVS/IRF3/STAT1 signailing pathway. (A) GSEA enrichment analysis results of dif ferential genes between ACSL4 high-expression and ACSL4 low-expression hepatoblastoma tissues in GSE133039 dataset. (B) Enrichment results for IFNα response via GSEA of the hepatoblastoma tissues in GSE133039 dataset. (C) Enrichment results for IFNγ response via GSEA of the hepatoblastoma tissues in GSE133039 dataset. (D) The enrichment results of the interferon-γ response signaling pathway between the ACSL4-high hepatoblastoma subgroup and the ACSL4-low hepatoblastoma subgroup in GSE186975 dataset. (E) The enrichment results of the interferon-γ response signaling pathway between ACSL4 knockdown HepG2 cell line and negative control cells. (F) The MAVS/IRF3/STAT1 interferon response pathway-associated proteins were detected by Western blot in ACSL4 knockdown cells and controls. (G) Inferred intercellular communication network among ACSL4 high tumor cells and other cell clusters, shown in a circle plot. The thickness of the lines represents the strength of cell communication. (H) Circle plots showing cell-cell communications between ACSL4 low tumor cells and other cell clusters

Article Snippet: Total RNA was extracted from ACSL4 knockdown and negative control lentivirus-infected HepG2 cells using the RNAstorm Fresh Cell and Tissue RNA Isolation Kit from Cell Data Sciences (USA).

Techniques: Expressing, Knockdown, Negative Control, Western Blot